Depending on the material used to make the cell culture bottle, there is also a distinction between glass and plastic. Here is a little bit of your own opinion. Different cells have different sensitivity to pancreatic enzymes, so in the experiment we often encounter some cells that have not digested for a long time, and the cell state has been declining until the final death and the progress of the experiment is not smooth. Of course, sometimes it can be achieved by adding some EDTA, etc. to enhance the ability of pancreatin, or to increase the concentration of trypsin. In the process of culturing some cells, I found that some cells have different adhesion to glass and plastic products, such as RAW264.7, smooth muscle cells, etc., after converting the culture flask, it can be found that it is well digested, and it does not change its adherence ability when returning to the culture bottle of the original product. Here I hope that experienced comrades can share your experience.
Cell culture equipment selection: In terms of size, cell culture flasks can be divided into about 600ml, 250ml, 50ml and 25ml , which are generally surface-modified, suitable for cell attachment and growth. 600ml and 300ml are mostly used for large-scale culture (such as culture of monoclonal cells), and 50ml is used for general cell experiments (general passage, preservation of cells, cells for experiments, etc.), 25ml is generally used. To resuscitate cells or cells with less cells, and to make multiple cells when making primary cells to avoid cross-contamination. Of course, there are other things, such as a circle, which can be chosen according to personal preferences and experiments.
As for the purchase of the culture plate, there is actually no experience. What I want to say is to avoid buying domestically produced culture plates. Some domestically produced culture bottles are OK, but the culture plates we bought a lot of domestically produced, the effect is not very good, the cells are The inside is basically very very embarrassing. Therefore, if you have enough money, you can buy these things if you can buy them. The basics of big companies can be bought, BD, Corning and so on. These things are in principle one-off, but most of our labs simply can't do it. In fact, soaking in acid, washing and reuse is no problem. After all, we don't have the wealth of foreigners, so we are tired, more Do some physical exercise and exercise your body.
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