Filler component Dionex HPLC column and flow phase should be required under each type items. Commonly used fillers are silica gel column chromatography and chemically bonded silica. The latter uses octadecylsilane-bonded silica gel Zui as the common octyl-bonded silica gel, and cyano or amino-bonded silica gel is also used; ion exchange fillers are used for ion exchange chromatography; gels or glass microspheres for molecules Exclusion chromatography, etc. The injection volume is generally a few microliters. Unless otherwise specified, the column temperature is room temperature and the detector is an ultraviolet absorption detector.
When using an ultraviolet absorption detector, the flow used corresponds to the solvent requirements under the ultraviolet spectrophotometry.
The conditions specified in the various items in the text shall not be arbitrarily changed except for the type of stationary phase, the composition of the mobile phase, and the type of detector. Others such as the inner diameter of the column, the length, the phase of the stationary phase, the particle size of the carrier, the flow rate of the mobile phase, and the mixed mobile phase. The proportion of the components, the column temperature, the injection amount, the sensitivity of the detector, etc., can be appropriately changed.
To adapt to specific varieties and meet the requirements of the system suitability test. The general chromatogram is recorded in about 20 minutes. 2. System suitability test
Applicability tests shall be carried out on the instrument according to the requirements of each product, that is, the instrument shall be tested and adjusted with the specified reference materials, and the specified requirements shall be met; or the number of theoretical plates, resolution and drag of the column shall be specified in the analytical state. Tail factor.
Electronic Door Lock,Tower Base Station Electronic Locks,Electronic Locking System,Electronic Door Locking System
Ningbo Hengchieh Locking Technology Co., Ltd. , https://www.yh-lock.com