Angelica's anti-inflammatory effects

Anti-inflammatory effect

1. Effect of xylene-induced ear swelling in mice.

2. Effect of acetic acid-induced increase in capillary permeability in the abdominal cavity of mice.

Mice were taken and randomly divided into 9 groups. One hour after the last administration, according to the literature method (WhittleBA.1964), iv 0.5% Evans blue 5ml/kg, immediately ip0.7% acetic acid 10ml/kg, 30 minutes after the mice were sacrificed, the abdominal cavity was cut, Rinse the saline repeatedly, collect the rinse solution, and adjust the final volume to 10 ml. Centrifuge and take the supernatant. Measure the absorbance at 610 nm using a 721 spectrophotometer. As a result, Angelica sinensis 10g/kg significantly inhibited the increased capillary blood permeability in the abdominal cavity.

3. Effects of inflammatory agents on paw swelling in rats.

Rats were randomly divided into groups of 7 feet. One hour after the last administration, the reference literature (Xu Shuyun et al., pharmacological experiment methodology) was used. Rats were given sc1% carrageenan, 2.5% formaldehyde, and fresh egg yolk, each with a hind paw pad. Measured once every hour (Formaldehyde group measured once every day 2 hours after administration, a total of 5 times, so that the difference in footpad thickness before and after inflammation indicates swelling degree. As a result, footpad swelling of 3 kinds of inflammatory agents in Angelica sinensis. All have significant inhibitory effects.

4. Effect of footpad swelling on carrageenan in adrenalectomized rats.

Rat adrenalectomy was performed according to conventional methods. Ether anesthesia was performed. A transverse incision was made under the dorsal ribs on the dorsal aspect of the abdomen. The side adrenal glands were removed. After the operation, penicillin 250 mg/kg was used for anti-infection and fed with 1% NaCl instead of tap water. On the second day, rats were randomized and administered simultaneously. One hour after the last administration, 0.1 ml of sc1% carrageenan was injected into the right hind paw, and the degree of swelling of the footpad was measured by the same method as above. Results; Angelica still had obvious anti-inflammatory effects on rats with adrenal glands.

5. Effect of increased histamine-induced capillary permeability in rat skin.

Rats were randomly divided into groups of 7 animals. One hour after the last administration, the back of the rat was shaved, and histamine phosphate 0.01 mg/0.05 ml was injected intradermally along both sides of the midline of the back. Immediately iv 0.5% Evans blue solution was applied at 10 ml/kg, and the locus was peeled off after 20 minutes. Skin, determination of blue spot dye exudation. As a result, Angelica sinensis had no significant effect on histamine-induced increase in capillary permeability.

6. Effect on PGE2 content in rat inflammatory tissues.

Rats were randomly divided into groups of 7 animals. 0.5 hour after the last administration, 0.1 ml of scl% carrageenan was injected into the right hind paw, and the rats were sacrificed 3 hours after the inflammation. The inflammatory foot pad was cut at 0.5 cm above the ankle joint, weighed, and peeled. Add 5ml saline to soak for 1 hour, remove the paw, centrifuge, and take the supernatant (Daiyue et al., Chinese Journal of Chinese Materia Medica, 1989; 14(7): 47) and measure the absorbance at 278nm using a 751 spectrophotometer. The PGE2 content was expressed as the equivalent absorbance per gram of inflammatory tissue. As a result, Angelica significantly reduced the content of PGE2 in the material. As a result, Angelica significantly reduced the PGE2 content in inflammatory tissues.

7. Effect on rat's cotton ball granulation tissue proliferation.

Female SD rats were randomly divided into 9 groups. Under ether anesthesia, 20±1mg sterilized cotton balls were implanted on both sides of the forelimb axilla. On the day of surgery, the rats were continuously dosed for 7 days. On the 8th day, the rats were sacrificed by bleeding and the granulation tissue was detached. The positive control was hydrocortisone 0.01 g/kg x 7 . After the granulation tissue was dried at 80°C for 3 hours and weighed, each rat's thymus and adrenal glands were weighed. As a result, Angelica 8g/kg group had a significant inhibitory effect on the granulation tissue proliferation of cotton balls, but had no significant effect on the weight of thymus and adrenal glands.

8. The effect of serum complement hemolytic activity in guinea pigs.

Guinea pigs were randomly divided into 7 groups. One hour after the last administration, the heart was aseptically bleed and serum was prepared. According to the modified Mayer's half-measure (Clinical and Biochemical Test, Hunan Science and Technology Press, 1981), the hemolytic activity of the serum-complementary classical pathway was measured. Half hemolysis value (ACH50) was calculated. According to the literature (Zhang Jifou et al., Anhui Medical College, 1985), serum complement complement hemolytic activity was measured. Half hemolysis value (ACH50) was calculated. As a result, Angelica had a significant inhibitory effect on complement hemolysis activity without affecting the hemolytic activity of the complement classical pathway.

The above studies suggest that the anti-inflammatory mechanism of Angelica sinensis mainly involves:

1. Reduce capillary permeability;

2. Inhibition of PGE2 synthesis or release.

In addition, reducing the hemolytic activity of guinea pig complement bypass may also be one of its anti-inflammatory mechanisms.

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