1 Determination of xylooligosaccharides (high performance liquid chromatography)
The method is suitable for the detection of low poly pool in health foods with added oligosaccharides.
1. 1 method summary
The components of oligosaccharides were quantitatively determined by high performance liquid chromatography. The separation order of the sugar on the carbohydrate analysis column with acetonitrile and water as the mobile phase is the monosaccharide followed by the disaccharide, which is first oligomerized and then polymerized, and detected by a differential refractive detector. From the product of this product is >95% of the low poly wood pond pure product, so the area ratio method can be used to determine the content of oligomeric germanium in the sample.
1. 2 instruments
(1) High performance liquid chromatography: Waters HPLC 515 pump, 410 differential refractive index detector, chromatographic work station.
(2) Ultrasonic oscillator.
(3) Microporous through the bay (filter membrane 0. 45um)
1. 3 reagents
(1) Acetyl (chromatographically pure)
(2) Water (three distilled water and treated with Milli-Q ultra-pure)
(3) D-xylose C5H1005 Shanghai Chemical Reagent Co., Ltd. (E. Merck Packing): Positioning. Because Sigma has no samples of xylooligosaccharides.
1. 4 determination steps
(1) Sample processing
Accurately weigh about 1.0g of the capsule contents with an accuracy of 0.001g. In a 100ml container, add water to about 80m1 and shake it for 15 minutes in an ultrasonic oscillator. Add water to the mark, shake well, and filter with 0.45um filter. Direct injection measurement.
(2) Chromatographic separation conditions:
Column: SUGAR PAK I 6.5MMX300MM COL, SUGAR-PAK INSERTS 10/PK, GUARD-PAK HOLDER
Mobile phase: acetonitrile + water (85+15)
Flow rate: 0.8m1/min
Side detector sensitivity: 4
Injection most: 20uL
(3) Sample determination:
A sample solution of 20 uL was injected into a high performance liquid chromatograph for separation. 1% pure xylose solution for positioning. According to the principle of separation of oligosaccharides, all peaks after the xylose peak are the sum of xylooligosaccharides. The content of xylooligosaccharides in the sample liquid was calculated from the peak area.
(4) The separation order of xylooligosaccharides is:
Arabinose (Al) grape vine (G1), xylose (X), xylobiose (X1), xylobiose (X2), Musitang (X3), xylofuran
(X4), wood hexose (X5), wood hexose (X6).
1.5 result calculation
One-hundred percent of oligosaccharides: Since each component is a homologue, the percentage of the total area of ​​each component of the oligosaccharide can be calculated by the area normalization method.
Xylooligo%=(x l+x2...x6/Al+G1+X+Xl... X6)x100
Where: A1: arabinose
G1: glucose
X: Hibiscus
X1-X6: Wood disaccharide - wood seven.
This method refers to:
1. The method for detecting the efficacy of health foods is edited by Wang Yaguang. (From Q/YJB0001s-2012)
Our company provides you with more low-poly xylose testing equipment, please contact us at 0571-61100054
Shanghai Rocatti Biotechnology Co., Ltd. is a professional enterprise of rehabilitation and medical device products with sales and service as the main body. Since its establishment, the company has been committed to the development of medical devices. Its products involve the production of epidemic prevention materials such as gynecology (obstetrics), Urology, neurosurgery, kn95 medical protective masks, daily use flat masks, and the research and development of total nutrition slimming food. It can provide a complete range of first, second and third class medical device configuration schemes for general hospitals and specialized hospitals.
Nonwoven Face Mask,Non-Woven Facial Face Mask,Non-Woven Face Mask Ffp2,Non-Woven Surgical Face Mask
Shanghai Rocatti Biotechnology Co.,Ltd , https://www.shljdmedical.com