Hong Kong's "Residues in Food Residues Regulations" overall solution and method package

Hong Kong's "Residues in Food Residues Regulations" overall solution and method package

Thermo Fisher Scientific Triple Quadrupole Qualitative

one. Method package introduction

The method package is a simple instrumental application process for the customer's needs by the application department of Thermo Fisher Scientific and Mass Spectrometry. The compounds involved in the method package are common compounds that can be detected on GC/MS, such as pesticide residues. Cycloaliphatic hydrocarbons, polychlorinated biphenyls, polybrominated biphenyls, polybrominated diphenyl ethers, phthalates, and the like.

The function of the method package is to enable customers to use the instrument faster and easier, and get started as soon as possible. The method package includes injection method, data processing method (TraceFinder method folder), related application articles, related standards, column information, pre-processing methods, data files, etc. The customer can directly call the injection method and data processing method to complete the compound. Qualitative and quantitative analysis.

Since the Thermo ScientificTM TSQ 8000 Evo uses the T-SRM injection method instead of the time-sharing method, the original injection method can be called directly, even if there is a slight deviation in retention time, and the final result will not be affected. In addition, the database provided by TraceFinder software can directly edit the data processing method. The database includes the name of the compound, ion pair, collision energy, quantitative ion, qualitative ion, CAS number and other information. At the same time, TraceFinder software can automatically correlate the method files that generate TSQ 8000 Evo according to the data processing method. This way, TraceFinder can be used to directly generate data processing methods and partial injection methods. The entire process is automated, requiring little operator input of any operational information.

Method Kit

For commonly used analytical compounds, we can provide a method package.

Including: phthalate, PCB, PAH, PBDE, pesticide residue screening, GB2763, pesticide residues in tobacco, PM2.5, Hong Kong regulatory program, nitrosamines, dioxins, etc.

Purpose: Easy to use, direct use.

two. Instrument introduction

TSQ 8000 Evo triple quadrupole series gas mass spectrometer

With a consistent lead in gas chromatography triple quadrupole mass spectrometry, Thermo Fisher Scientific has reinvented the TSQ 8000 after the introduction of the TSQ 8000 Evo, a highly efficient GC/MS. /MS delivers never-ending productivity with exceptional sensitivity, ultra-fast scanning speeds, and simple MSMS capabilities to meet the most demanding quantitative analysis requirements, providing the foundation for food safety, environmental analysis, forensic and pharmaceutical applications.

TSQ 8000 Evo main features:

• AutoSRM function automatically optimizes secondary ion pair information;

• Timed Retention Time SRM function (T-SRM) and Timed Retention Time SIM function (T-SIM) make high-throughput detection possible, and the optimization method sets parameters without assigning time periods;

• The latest TraceFinder software, which provides the latest technology platform and user-friendly chromatographic analysis software, can directly call the database information to automatically establish data processing methods and injection methods;

• Up to 800 MRM/s scanning speed, even without extreme loss of sensitivity under extreme high-speed scanning conditions;

• Replacing the entire ion source while the mass spectrum is not venting, saving on routine system maintenance and simplifying operation;

• Double filament design with filaments on the same side;

• Double heating zone on the ion source to effectively remove the contamination of the ion source by the substrate and save maintenance time;

• Curved pre-quadpoles effectively remove neutral noise, reduce background and increase sensitivity.

three. Compound introduction

The Hong Kong Pesticide Residues in Food Regulation contains 360 pesticides and a maximum residue/maximum residue limit of more than 7,000 “pesticide-food” combinations. Pesticides, also known as pesticides, are commonly used to kill or control harmful insects, plants, fungi or other harmful organisms, as well as some growth regulators. In the food, the use of pesticides, or the incorporation of pesticides in the feed, or the presence of pesticides in their own environment may migrate into the food, which requires the detection of these harmful substances.

The pesticides included in the Hong Kong Regulations can detect more than 95% of the compounds by GC/MS/MS and LC/MS/MS, and Thermo Fisher Scientific can already provide a comprehensive GC/MS/MS and The overall solution for LC/MS/MS.

The figure below is a list of compound information completed on GC/MS/MS.

Remarks:

Standard sample purchase: Sigma-Aldrich company, BEHRINGER, Quandao, Zhenxiang, Anpu, etc. are provided with standard samples.

four. Related regulations and policies

The procedures for reviewing the Residues of Pesticides in Food Regulations (Cap 132CM) (the Regulations) were completed in June 2012. The relevant regulations will be implemented as of August 1, 2014 (1). The Residues in Pesticides Regulations on Foods sets out the maximum allowable levels of pesticide residues in certain foods and some interpretation provisions. They are the regulations and policies that must be followed for vegetable projects in Hong Kong. The compounds tested in the Regulations and the limit standards are mainly supplemented by the relevant standards available at the time in the Mainland and other countries that export food to Hong Kong, taking into account the views collected from public consultations in July-September 2011, and these standards have been Passed a risk assessment.

reference:

1. http://

Fives. Sample preparation

The current detection methods are aimed at more and more pesticides, and the types of substrates involved are various. Therefore, QuEChERS (Quick, Easy, Cheap, Effective, Ruggedand Safe) is a relatively common and effective pretreatment method. Health. The QuEchERS method has a high recovery rate of a large number of polar and volatile pesticides, high analytical accuracy, simple operation and low pollution, and is therefore widely used in the large-scale sample processing detection of the vegetable supply project in Hong Kong. The QuEchERS method is briefly summarized as the process of extraction, purification and extraction, as shown in the figure below (refer to EN 15662 method):

six. Instruments and equipment

Instrument: Thermo ScientificTM TSQ 8000 Evo Gas Chromatography Triple Quadrupole Tandem Mass Spectrometer.

Equipment: vortex mixer, centrifuge, rotary evaporator, nitrogen blower, etc.

Column: TR-Pesticide II (30 m*0.25 mm*0.25 μm with 5 m precolumn) capillary column (Thermo Fisher Scientific). The C10 retention time is 18.90 min.

Seven. Method package use

1. Injection method use: directly use the file ending in meth in the method package , for injection analysis.

Different instrument configurations may vary slightly. You can refer to the screenshot below.

2. Data processing method creation: directly copy the folder in the method package To C:\TraceFinderData\32\

In Methods, methods can be opened directly in the TraceFinder software. This method includes data processing methods and injection methods.

Copy and paste the injection method from step 1 into C:\TraceFinderData\ InstrumentMethods, select this file in Instrument Methods, and save the TraceFinder Master Method.

2. Associate the data file completed in step 1 with the TraceFinder method to get the complete data processing method. Parameters such as retention time are changed as needed to further optimize the data processing method to obtain an accurate data processing method. The whole process does not need to input compound information, ion pair information, select ion pair information to find compounds, all steps are automatically completed, one point can be, greatly improving the analysis efficiency and simplifying the method flow.