Luminescence reflects the most basic processes in biological, chemical, and physical changes. With the development of photon detection technology, it has been found that in addition to general bioluminescence and chemiluminescence, all living organisms have a very weak photon radiation called ultra-weak luminescence. Its spectrum is in the range of 180-800 nm, the light intensity is very weak, only 10-104 photons/sec·cm2, and the quantum efficiency is 10 -14 -10 -9 . Although the light intensity is very weak, the general luminescence phenomenon of biological organisms is intrinsically linked to the processes of oxidative metabolism, detoxification, cell division and death, photosynthesis, carcinogenesis and growth regulation. Recently, many biologists and medical scientists at home and abroad have applied this luminescence measurement to applications such as medical diagnosis. For example, through the luminescence measurement of the organism itself, the relationship between the survival of bacteria, algae and microorganisms and its environmental factors can be judged, and the environmental pollution can be evaluated by the regularity study of the basic characteristics of the spontaneous emission of the living organism. Measuring the luminescence of blood and certain tissues helps to diagnose the disease. This is an emerging field of technology that has unique advantages over general luminescence measurements. The BPCL Weak Luminance Meter can meet a variety of measurements through research and extension applications.
First, existing techniques for analyzing luminescence:
Ultra-weak luminescence analysis: Any living matter, human, animal, plant and microbe has a kind of ultra-weak luminescence, which is an attribute inherent in the living body itself. This luminescence can be achieved by biological macromolecules, cells, subcellular components (mitochondria, microsomes, liposomes) or living organisms (mouse, leaves, fruits, small fish), organs and body fluids (blood, urine). The measurement is detected.
Bioluminescence analysis: It has been developed into a bacterial luminescence test system represented by luciferase-luciferase, such as the analysis of the effect of anesthetics on the human body by the luminescence of bacteria, the residual amount and titer of antibiotics in milk and blood, and The number of bacteria in the urinary tract infection, etc., is determined by measurement of ATPase in bacteria.
Analysis of free radicals and reactive oxygen species: the generation and change of free radicals and reactive oxygen species are always accompanied by the emission of photons. Luminescence detects free radicals and reactive oxygen species, and its sensitivity can be compared to the ESR method.
Chemiluminescence analysis: When a chemical reaction occurs between chemical substances, part of the chemical energy is always emitted in the form of light. The detection of the change law of chemiluminescence is a powerful means to study various chemical reactions and biochemical reactions.
Luminescence immunoassay: Luminescence measurement can be used to measure the body's hormones, vitamins, drugs and trace elements, and its sensitivity and specificity can be compared with radioimmunoassay.
Environmental monitoring: The degree of water pollution can be monitored macroscopically by inhibiting the luminescent bacteria by harmful substances in the water. At the same time, there are also studies on the monitoring of harmful substances in the air by gas sensors.
Agroforestry application: According to the luminous intensity of plant seedlings, the cold resistance, heat resistance, salt tolerance and nutrient development of plants are judged, which provides a basis for agricultural breeding and cultivation techniques.
Second, the advantages of luminescence analysis application
The application of luminescence analysis method has many advantages, the method has high sensitivity, low detection limit, simple sample pretreatment, fast analysis speed, and the advantages of miniaturization and portability of the instrument.
Third, method development and application cooperation
As the basic research of the illuminating method continues to deepen, the application range is continuously expanded, and the illuminating method has the advantages of fast analysis speed and high sensitivity, and has been widely accepted throughout the world after several decades of precipitation. The low-light technology has mastered the core photoelectric conversion technology, and can completely develop new analytical instruments according to actual analysis requirements, and commercialize them for the benefit of society. If you have mastered the analytical test method, and the market needs this method to solve the analytical test problem, and encounters no off-the-shelf analytical instruments, you can consider cooperating with the micro-light technology to develop together. From a complete team of mechanical design, electronics, optical engineering and software development, we look forward to working with you to create a better future.
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