In order to find out the effect of 5% d-limonene solution on the control of citrus scorpion (Citrus red spider), researchers from Zhejiang University College of Agriculture and Biotechnology conducted indoor egg suppression test and different insect base and temperature. Field control trials under conditions. The results showed that 5% d-limonene solution could significantly inhibit the hatching of citrus scorpion eggs, and the survival rate of larvae after hatching decreased, and it had better control effect on citrus catfish in the field.
It is recommended to spray 5% d-limonene solution 200-300 times liquid spray or 5% d-limonene solution 500 times liquid in the low citrus base (2~3 heads/leaf) or spring occurrence period. 24% snail ester 5,000 times solution spray treatment, in the citrus scorpion mites high base (9 ~ 10 head / leaf) or autumn occurrence is recommended 5% d-limonene solution 200 ~ 300 times solution + 24% Spirulina 5,000 times solution spray treatment.
According to reports, d-limonene is an orange oil extracted from orange peel by professional cold pressing technology, which belongs to natural plant-derived pesticides. The action mode of pests is a unique physical contact effect, and it has no cross-resistance with common chemical pesticides. The insecticidal mechanism is to dissolve the waxy layer of the body surface of the pest, so that the pests are quickly knocked down and die in a state of obvious water loss. As of the end of April 2018, the registered crops and control objects for the approval of d-limonene products in China are tomato whitefly (excluding sanitary insecticides).
This article URL: d-limonene has a better effect on the control of citrus
The RNA purification kit is used to purify and recover RNA molecules transcribed in vitro and total RNA extracted from various materials, which can effectively remove contaminating impurities in RNA samples. The recovery rate of this product can reach 80%, and the OD260/OD280 ratio of the obtained RNA is generally about 2.0, which can be directly used in subsequent sensitive experiments (such as microarray analysis, fluorescence RT-PCR, etc.). With the deepening of transcriptomics, the complexity of RNA types, expression regulation and functions is far beyond our imagination.
Removing ribosomal RNAs that account for more than 80% helps to focus sequencing on less abundant but informative RNAs. At present, the removal of rRNA is mainly through the combined use of probe and RNase H. The processed RNA will be mixed with many digestion products, enzymes and ions, which is not conducive to the subsequent construction of RNA library. Take the Columnar RNA Purification Kit as an example. Trizol is a ready-to-use reagent that can be used to purify total RNA from tissues and cells. This is a single-phase solution of phenol and guanidine isothiocyanate that facilitates lysis of tissues and cells, inhibiting RNases to maintain RNA integrity.
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