First, nutrition
Although Pleurotus ostreatus is a wood saprophytic fungus, its requirements for culture materials are not strict. As long as it has the nutrient elements needed for the growth and development of Pleurotus ostreatus, it can be used to cultivate Pleurotus ostreatus, such as wood chips, cottonseed hulls, waste cotton, and corn. Raw materials such as core, straw, and distiller's grains are properly treated to achieve a suitable carbon-nitrogen ratio, which can be used to cultivate oyster mushrooms. It is generally believed that the ratio of carbon to nitrogen in the vegetative growth stage of Pleurotus ostreatus is 20:1, and that in the reproductive growth stage is 40:1. Accordingly, in order to increase the yield of Pleurotus ostreatus, the nitrogen source is appropriately supplemented. Inorganic nitrogen source mainly refers to nitrate, ammonium salt and other substances, but it is not suitable to add more, otherwise it will inhibit mycelial growth; organic nitrogen sources mainly include bran, rice bran, corn flour, soy flour, human and animal waste. The culture material to which the organic nitrogen source is added needs to be sterilized or accumulated for fermentation before being used for cultivation. Avoid adding organic nitrogen sources when planting raw materials, otherwise it will be susceptible to infection and lead to pests and diseases.
Second, the temperature
Temperature is an important environmental factor affecting the natural occurrence of Pleurotus ostreatus. The temperature range of Pleurotus ostreatus is 3 °C-35°C, slow growth below 7°C, faster growth above 15°C, and the highest temperature for mycelium growth. It is 24-28 ° C. The suitable temperature for the differentiation and development of fruiting bodies varies with the variety. The formation of low temperature type fruiting bodies requires 12-15 °C, the medium temperature type needs 16-22 °C, and the high temperature type requires 20-28 °C. The broad temperature type seed body is formed. The temperature range is wider.
Pleurotus ostreatus is a temperature-changing, solid mushroom, which requires stimulation of temperature changes when the fruit body is formed. In the specific cultivation, it should be flexibly controlled according to different varieties. If raw material cultivation is adopted, since the culture material contains more microorganisms, the breathing is fast and the heat production is the largest, and the temperature of the material is often raised. When planting, the principle of “lower and lower than high†should be adopted. The suitable ambient temperature should be around 20 °C. In addition, in the temperature range of growth of fruiting bodies, when the temperature is low, the fruiting body grows slowly, but the mushroom is thick; when the temperature is high, although the mushroom body matures quickly, the mushroom cover is thin and the quality is poor. Therefore, understanding the quality of the oyster mushrooms is one of the keys to the cultivation of oyster mushrooms.
Third, moisture and humidity
Pleurotus ostreatus is a moist-loving mushroom. The growth of oyster mushrooms requires water, and most of it comes from culture materials. Therefore, the water content of the culture material is an important factor affecting mycelial growth and mushrooming. The moisture content of the culture material in the germination period should be controlled within the range of 60-65%, which varies slightly depending on the cultivation materials and cultivation methods. Pleurotus ostreatus generally adopts closed cultivation management during the germination period. At this stage, the air humidity requirement is not strict, generally about 60-70%. Pleurotus ostreatus primordium differentiation and fruiting body development period, metabolic activity is very strong, coupled with open management, it is required to increase the relative humidity of the air, generally in the air relative humidity of 85-95%, can form a strong and tender fruiting body .
Fourth, the air
Pleurotus ostreatus has different requirements for oxygen and carbon dioxide at different stages of growth. According to research, in the mycelial growth stage of Pleurotus ostreatus, if the concentration of carbon dioxide is between 20-30%, the growth amount is 30-40% higher than that under normal air. When the concentration of carbon dioxide is more than 30%, the mycelial growth is obviously inhibited. Therefore, in the growth stage of Pleurotus ostreatus, it is more resistant to carbon dioxide, and the plastic film is covered on the surface to create a relatively closed environment. The hyphae are in a semi-anaerobic state, so that other aerobic bacteria are not easy to grow and multiply, and the purpose of promoting hyphal growth, preventing contamination of bacteria and moisturizing can be achieved. However, semi-anaerobic is not equal to anaerobic. Therefore, when covering with a film, a needle should be used to puncture a hole on the film to form a microporous effect, so that there is an appropriate amount of air exchange, which is advantageous for the growth of mycelium for management. When the mycelial growth is mature, it is necessary to increase the ventilation to meet the oxygen demand of primordium differentiation and fruit body development. At this time, if the ventilation is poor, it will cause the scallop mushroom body to have a long handle, a small cover, and even a deformed mushroom. The three environmental factors of temperature, humidity and air should be considered comprehensively and coordinated, and we must not lose sight of it.
Five, lighting
In the mycelial growth stage, darkness is conducive to mycelial growth; in the mushrooming stage, proper astigmatism must be given, and the primordial group can occur. Therefore, during the mycelial growth stage, care should be avoided as much as possible. Timely transmission of astigmatism to promote primordial differentiation. In this way, the mushrooms can be neatly arranged, the quantity is large, and the output is high.
Sixth, pH
Pleurotus ostreatus, like other wood rot fungi, prefers to be on acidic cultures with a pH between 5.8 and 6.2, and the mycelium is best. The commonly formulated culture material has a natural pH that meets the requirements for mycelial growth without testing and conditioning. In the growth process of Pleurotus ostreatus, the pH of the culture material is lowered due to the acid production by metabolism. In order to prevent the contamination of the bacteria during the cultivation, the neutralization of the mycelium metabolism produces acid, and the pH of the culture material is required to be slightly higher.
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